This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
Anti-5HT3A Receptor Rabbit mAb
Catalog number :AT0434
- Overview
- Description
- Rabbit monoclonal antibody to 5HT3A receptor
- Reactivity
- Mouse, Rat, Human
- Tested applications
- WB: Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 55 kDa.
IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- Properties
- Immunogen
Synthetic peptides, corresponding to amino acids 21-36 and 421-436 of Rat 5HT3 Receptor.
- Clonality
- Monoclonal, clone number: 2EA7
- Isotype
- Rabbit IgG
- Form
- Liquid
- Storage instruction
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Database links
- Entrez Gene: 3359 Human
- Entrez Gene: 15561 Mouse
- Entrez Gene: 79246 Rat
- Omim: 182139 Human
- SwissProt: P46098 Human
- SwissProt: P23979 Mouse
- SwissProt: P35563 Rat
- Unigene: 413899 Human
- Applications
- WB Image
All lanes : Anti-5HT3A receptor antibody (AT0434) at 3 µg/ml
Lane 1 : Human Brain tissue lysate
Lane 2 : Mouse Brain tissue lysate
Lane 3 : Rat Brain tissue lysate
Predicted band size : 55 kDa
- IHC Image
IHC image of AT0434 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with AT0434, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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