Anti-ALIX Rabbit mAb - Exosome Marker

Product

Anti-ALIX Rabbit mAb - Exosome Marker

Catalog number :AT1610

Background:

Alix, a phylogenetically conserved cytosolic scaffold protein, contains an N-terminal Bro1 domain, a coiled-coil region and a C-terminal proline-rich domain. Originally identified as an ALG-2 (apoptosis-linked gene 2)-interacting protein involved in programmed cell death, Alix also regulates many other cellular processes, such as endocytic membrane trafficking and cell adhesion through interactions with ESCRT (endosomal sorting complex required for transport) proteins, endophilins, and CIN85 (Cbl-interacting protein of 85 kDa).

Alix has also been shown to be involved in the biogenesis of exosomes, small secreted vesicles that contribute to cell signaling. In addition, Alix interacts with Atg12-Atg3 to promote autophagy and endosomal fusion

UniProt ID: Q8WUM4

Overview

Reactivity: Human, Mouse, Rat, Bovine, Pig, Monkey

Tested applications: Western Blotting: 1/1000-1/2000

Immunofluorescence: 1/500

Immunohistochemistry (Paraffin): 1/500

Immunoprecipitation(IP) : 1/100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects endogenous levels of total Alix protein.

Properties

Immunogen: a synthetic peptide corresponding to residues surrounding Pro346 of human Alix protein.

Clonality: Monoclonal, clone number: 6B7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Alix Antibody- Analysis of ALIX on different cell lysates using anti-ALIX antibody at 1/1,000 dilution.Positive control:Lane 1: Hela Lane 2: PC-12Lane 3: NIH-3T3

IHC Image: Immunohistochemistry-Paraffin: Alix Antibody - Analysis of paraffin-embedded human colon cancer tissue using anti-ALIX antibody. Counter stained with hematoxylin.

ICC/IF Image: Genetic Strategies Validation. Immunocytochemistry/Immunofluorescence: Alix Antibody - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.

Anti-ALIX Rabbit mAb - Exosome Marker

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