Anti-ALK Rabbit mAb

Overview

Description

Rabbit monoclonal antibody to ALK

Reactivity

Human, Mouse, Rat

Tested applications

WB: 1/50 - 1/100. Predicted molecular weight: 176 kDa.

IHC-P: 1/10 - 1/50.

Properties

Immunogen

KLH conjugated synthetic peptide selected from the Central region of human ALK.

Clonality

Monoclonal, clone number: 4F11

Isotype

IgG

Form

Liquid
Preservative: 0.09% Sodium Azide
Constituents: PBS

Storage instruction

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Database links

• Entrez Gene: 238 Human
• Omim: 105590 Human
• SwissProt: Q9UM73 Human
• Unigene: 654469 Human

Applications

WB Image

Western blot - ALK antibody

All lanes : Anti-ALK antibody (AT0424) at 1/50 dilution

Lane 1 : 293 cell lysate, non transfected
Lane 2 : 293 cell lysate transiently transfected with the ALK gene

Lysates/proteins at 2 µg per lane.


Predicted band size : 176 kDa
Observed band size : 250 kDa

Why is the actual band size different from the predicted?

Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include...

• post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein
• post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases
• splice variants - alternative splicing may create different sized proteins from the same gene
• relative charge - the composition of amino acids (charged vs non-charged)
• multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands

IHC Image

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ALK antibody

AT0424 at 1/50 dilution staining ALK in human testis tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.