Anti-AMPK alpha 1 Rabbit mAb

Product

Anti-AMPK alpha 1 Rabbit mAb

Catalog number :AT0352

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Overview

Description: Rabbit monoclonal antibody to AMPK alpha 1

Reactivity: Mouse, Rat, Cow, Human

Tested applications

IHC-P	: Use a concentration of 5 µg/ml.
ICC/IF	: Use a concentration of 1 µg/ml.
IP	: Use at 2-8 µg/mg of lysate. Recommended to be used at a concentration of 2 - 8 µg/mg of lysate.
WB	: 1/500 - 1/5000. Predicted molecular weight: 63 kDa.

Properties

Immunogen: Synthetic peptide (Human) - which represented a portion of AMP activated protein Kinase (AMPK), alpha 1 catalytic subunit encoded within exon 8.

Clonality: Monoclonal, clone number: 5SA7

Isotype: IgG

Form: Liquid
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8

Storage instruction: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Applications

WB Image: Western blot - AMPK alpha 1 antibody

Predicted band size : 63 kDa

Samples: Extracts from

1. Bovine aortic endothelial cells

2. Rat aortic smooth muscle cells

3. HepG2 cells

4. Human aortic endothelial cells

Antibody: Affinity purified Rabbit anti-AMPK alpha 1 (AT0352) used at 2ug/ml (1/500).

IHC Image: For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-AMPK alpha 1 Rabbit mAb

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