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    Anti-AMPK alpha 2 Rabbit mAb

    Catalog number :AT0610
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    AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia, and ischemia. The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKα at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation. AMPKα is also phosphorylated at Thr258 and Ser485 (for α1; Ser491 for α2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated. The β1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182. Phosphorylation at Ser108 of the β1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization. Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle. Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS.
    Overview
    Reactivity
    Mouse, Rat, Human
    Tested applications
    Western Blot 1:500-1:3000
    ELISA 1:100 - 1:2000
    ICC/IF 1:500
    Immunohistochemistry-Paraffin 1:100-1:1000
     
    Specificity
    AMPKα2 Antibody detects endogenous levels of total AMPKα2. The antibody does not cross-react with AMPKα1.
    Properties
    Immunogen
    Recombinant protein encompassing a sequence within the center region of human AMPK alpha 2. The exact sequence is proprietary.
    Clonality
    Monoclonal, clone number: 5F7
    Isotype
    Rabbit IgG
    Form
    Liquid, 100 μl,1mg/ml
    Storage instruction
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C
    Applications
    WB Image

    Western Blot: AMPK alpha 2 Antibody - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE; antibody diluted at 1:1000.
    IHC Image

    Immunohistochemistry-Paraffin: AMPK alpha 2 Antibody - Paraffin-embedded mouse brain, using antibody at 1:500 dilution.

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