Anti-APC Rabbit mAb

Overview

Description

Rabbit monoclonal antibody to APC

Reactivity

Mouse, Human, Xenopus laevis

Tested applications

ICC/IF: Use a concentration of 1 µg/ml.
WB: Use at an assay dependent dilution. Predicted molecular weight: 312 kDa.
IP: Use at an assay dependent dilution.
IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Properties

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (Human)(C terminal)

Clonality

Monoclonal, clone number: 2G7

Isotype

IgG

Form

Liquid
Preservative: Sodium Azide
Constituents: BSA, 10mM PBS, pH 7.4

Storage instruction

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Database links

• Entrez Gene: 324 Human
• Entrez Gene: 11789 Mouse
• Entrez Gene: 24205 Rat
• Entrez Gene: 399455 Xenopus laevis
• Omim: 611731 Human
• SwissProt: P25054 Human
• SwissProt: Q61315 Mouse
• SwissProt: P70478 Rat

Applications

IHC Image

 
Normal human colon section stained with AT0391 (1:100 for 10 min at RT). Sections were pre-treated by boiling in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

ICC/IF Image

ICC/IF image of AT0391 stained A431 cells. The cells were 4% paraformalehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (AT0391, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.