Anti-APG5L/ATG5 Rabbit mAb

Product

Anti-APG5L/ATG5 Rabbit mAb

Catalog number :AT0784

Background:

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

UniProt ID: Q9H1Y0

Overview

Reactivity: Human, Mouse, Rat, Bovine, Pig, Monkey

Tested applications: Western Blotting: 1/1000-1/2000

Immunofluorescence: 1/500

Immunohistochemistry (Paraffin): 1/500

Immunoprecipitation(IP) : 1/100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects endogenous levels of total Atg5 protein. This antibody is capable of detecting Atg5 conjugated to Atg12.

Properties

Immunogen: a synthetic peptide corresponding to residues surrounding Leu265 of human Atg5 protein.

Clonality: Monoclonal, clone number: 5A8

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: ATG5 Antibody - Analysis in mouse wildtype ES cell lysate (Lane 1) using This antibody. Lane 2 is a mouse ATG5 KO ES cell lysate (negative control).

IHC Image: Immunohistochemistry: ATG5 Antibody - Staining of human liver hepatocytes at 2.5ug/ml. 40X magnification.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: ATG5 Antibody - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-ATG5 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue).

Anti-APG5L/ATG5 Rabbit mAb

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