Anti-ATM (phospho S1981) Rabbit mAb
Catalog number :AT0639
Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair. Activation of ATM by autophosphorylation on Ser1981 occurs in response to exposed DNA double stranded breaks. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair. Known substrates include p53, Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, H2A.X, SMC1, FANCD2, Rad17, Artemis, Nbs1, and the I-2 regulatory subunit of PP1. Mutations in the corresponding ATM gene result in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation, and a higher frequency of chromosome breakage.
- Overview
- Reactivity
- Mouse, Rat, Human
- Tested applications
- Western Blotting 1:1000Immunoprecipitation 1:50Immunohistochemistry (Paraffin) 1:500
- Specificity
- This antibody recognizes endogenous levels of ATM protein only when phosphorylated at Ser1981.
- Properties
- Immunogen
- Synthetic peptide (Human) with a phosphorylated serine (1981)
- Clonality
- Monoclonal, clone number: 12C7
- Isotype
- Rabbit IgG
- Form
- Liquid, 100 μl,1mg/ml
- Storage instruction
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C
- Host
- Rabbit
- Applications
- WB Image
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog: AT0639) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog: AT0097). A specific band for Phospho-ATM (S1981) was detected at approximately 370 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda phosphatase (lambda -PPase) for 1 hour.
Related Products
Reviews
loading...
Payments by 
All our products are For Research Use Only. Not for diagnostic or therapeutic usages.