Anti-ATPB Rabbit mAb - Mitochondrial Marker

Product

Anti-ATPB Rabbit mAb - Mitochondrial Marker

Catalog number :AT0811

Background:

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha beta subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

SwissProt: P06576 Human

Overview

Reactivity: Mouse, Rat, Human, Monkey, Pig, Zebrafish

Tested applications: Western Blotting: 1/1000-1/5000

Immunofluorescence: 1/500

Immunohistochemistry (Paraffin): 1/500

Immunoprecipitation(IP) : 1/100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total complex V beta subunit (ATPB) protein.

Properties

Immunogen: This antibody was developed against Recombinant Protein

Clonality: Monoclonal, clone number: 4W2

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: ATPB Antibody - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

IHC Image: Immunohistochemistry-Paraffin: ATPB Antibody - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: ATPB Antibody - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.

Anti-ATPB Rabbit mAb - Mitochondrial Marker

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