Alpaca-HRP-IgG2a™ Anti-Mouse IgG2a (heavy chain) Alpaca Nanobody (HRP)

Product

Alpaca-HRP-IgG2a™ Anti-Mouse IgG2a (heavy chain) Alpaca Nanobody (HRP)

Catalog number :AT8302B

This antibody is UNIQUE,

Alpaca-HRP-IgG2a™ reacts with Mouse IgG2a (heavy chain) selectively, no reactivity with light chain of mouse IgG.

it can be used for Co-Incubation of primary antibody and secondary antibody in WB. it can save 1 hour to 2 hours in WB.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Alpaca immunoglobulin. The size of VHH is extremely small (<15 kDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus, VHH is considered of great value for research, diagnostics and therapeutics.

Alpaca-HRP-IgG2a™ Anti-Mouse IgG2a (heavy chain) Alpaca Nanobody (HRP) is designed for detecting IgG2a heavy chain of Mouse IgG specifically. Alpaca-HRP-IgG2a™ is based on monovalent, recombinant single domain antibodies coupled to HRP. In western blot and/or ELISA, Alpaca-HRP-IgG2a™ reacts with Mouse IgG2a (heavy chain) selectively, no reactivity with light chain of mouse IgG, and no reactivity with rabbit, human, cynomolgus, rat, goat IgG.

Overview

Description: Alpaca-HRP-IgG2a™ Anti-Mouse IgG2a(heavy chain) Alpaca Nanobody conjugated HRP

Reactivity: Alpaca-HRP-IgG2a™ reacts with Mouse IgG2a(heavy chain) selectively, no reactivity with light chain of mouse IgG.

and no reactivity with rabbit, human, cynomolgus, rat, goat IgG.

Tested applications: ELISA: 1:2000-1:10000

WB: 1:2000-1:10000

Properties

Immunogen: IgG2a (heavy chain) of Mouse IgG

Isotype: VHH domain of alpaca

Conjugation: HRP

Host: Alpaca pacous

Storage buffer: 10mM PBS (pH 7.5), 0.01% proclin300, 50% Glycerol

Purity: Recombinant Expression and Affinity purified

Applications

Highlights: Benefits Of Alpaca Nanobody

No Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments

Highly specific binding

Low background

High affinity

High stability

Alpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cells

Animal-free production, recombinant expression in yeast and affinity purified

Alpaca-HRP-IgG2a™ Anti-Mouse IgG2a (heavy chain) Alpaca Nanobody (HRP)

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