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    Annexin V-APC Apoptosis Analysis Kit (APC-AnnexinV/PI)

    Catalog number :IF8561
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    Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled Annexin V in a calcium-dependent manner.

    In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD. These cells will stain with Annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late stage apoptosis, the cell membrane loses integrity thereby allowing Annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (Annexin V, viability dye-positive) from the early-stage apoptotic cells (Annexin V positive, viability dye-negative).
    Overview
    Description
    APC Annexin V Apoptosis Detection Kit with PI
    Reactivity
    All mammalian species
    Tested applications
    Flow cytometry and fluorescence microscopy
    Properties
    Components
    Annexin V APC
    Binding Buffer
    Propidium Iodide Staining Solution
    Storage instruction
    Store between 2°C and 8°C. Do not freeze. 
    Caution: PI is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
    Applications
    Application Image


    Jurkat cells were either untreated (left) or treated for 4 hours with 10 uM camptothecin (right) and then stained with the Annexin V Apoptosis Detection Kit APC and Propidium Iodide Staining Solution (cat.no: CA4205). The Annexin V+ PI- population contains early stage apoptotic cells.

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