Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled Annexin V in a calcium-dependent manner.
In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD. These cells will stain with Annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late stage apoptosis, the cell membrane loses integrity thereby allowing Annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (Annexin V, viability dye-positive) from the early-stage apoptotic cells (Annexin V positive, viability dye-negative).
Annexin V-APC Apoptosis Analysis Kit (APC-AnnexinV/PI)
Catalog number :IF8561
- Overview
- Description
- APC Annexin V Apoptosis Detection Kit with PI
- Reactivity
- All mammalian species
- Tested applications
- Flow cytometry and fluorescence microscopy
- Properties
- Components
- Annexin V APC
Binding Buffer
Propidium Iodide Staining Solution
- Storage instruction
- Store between 2°C and 8°C. Do not freeze.Caution: PI is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
- Applications
- Application Image
Jurkat cells were either untreated (left) or treated for 4 hours with 10 uM camptothecin (right) and then stained with the Annexin V Apoptosis Detection Kit APC and Propidium Iodide Staining Solution (cat.no: CA4205). The Annexin V+ PI- population contains early stage apoptotic cells.
Related Products
Reviews
loading...