Annexin V-FITC Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. The kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining.
Annexin V-FITC Apoptosis Analysis Kit (FITC-AnnexinV/PI)
Catalog number :IF8560
- Properties
Product name Annexin V-FITC /PI Apoptosis Detection Kit Tests 100 x 1 assay Sample type Sample type Assay type Direct Assay time 10m Tested applications Fluorescence Microscopy, Flow Cyt Images 
Annexin V-FITC/ PI staining of AG06173 primary fibroblasts.
5 cells were used for analysis. Resuspended cells were incubated with Annexin V-FITC for 15 min in the dark. Propidium iodide was used as a counterstain to discriminate necrotic/ dead cells from apoptotic cells.Left: negative control - AG6173 untreated cells. Right: positive control - AG6173 cells irradiated at 10 Gy.
Related Products
Reviews
loading...
Payments by 
All our products are For Research Use Only. Not for diagnostic or therapeutic usages.