Anti-FOXP4 antibody - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-FOXP4 antibody - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :ATC3001

FoxP4 (Forkhead box protein P4; also FKHLA) is a 70-80 kDa member of the winged helix transcription factor gene family, FoxP subfamily of proteins. It is a transcriptional repressor that is strongly expressed in embryonic gut and lung epithelium, and in neurons both pre-and postnatally. Postnatally, it is found in cerebellar Purkinje cells. During development, it is found in hippocampal hilus, proliferating cells of the ventricular zone, and neurons of the basolateral nucleus of the amygdala. Human FoxP4 is 680 amino acids (aa) in length. It contains a large poly-Gln region (aa 65-219), a C2H2 Zn-finger domain (aa 307-332), a Leu-zipper dimerization motift (aa 349-370) and a forkhead DNA binding domain (aa 467-559). Phosphorylation occurs at Ser360. FoxP4 forms homodimers, and heterodimers with FoxP1 and FoxP2; it does not bind CTBP1. One potential splice form shows a deletion of aa 384-395. Over aa 549-680, human and mouse FoxP4 share 89% aa identity.

Overview

Description: Anti-FOXP4 rabbit monoclonal antibody - ChIP, CUT&RUN and CUT&Tag Grade

Reactivity: human,mouse,rat

Tested applications: WB: 1:1000
IHC: 1:200
ICC/IF:1:200
COIP: 1:50

ChIP: 10-20ul per reaction

CUT&RUN: 2-10ul per reaction

CUT&Tag: 2-10ul per reaction

Specificity: Recognize FOXP4 specifically, do not recognize FOXP1, FOXP2 and FOXP3.

Properties

Immunogen: Synthetic peptide of human FOXP4

Clonality: Monoclonal, clone number: 4D8

Isotype: rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol,0.02% Sodium Azide

Storage instruction: Store at -20°C, Avoid freeze / thaw cycle.

Database links: Q8IVH2

Host: rabbit

Applications

WB Image: Western blot shows lysates of COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line either mock transfected, transfected with human FoxP1, transfected with human FoxP2, transfected with human FoxP3, or transfected with human FoxP4. PVDF membrane was probed with 1:1000 dilution of Anti-FoxP4 Antibody (Catalog # ATC3001) followed by HRP-conjugated Anti-rabbit IgG Secondary Antibody.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: FoxP4 Antibody - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.

Anti-FOXP4 antibody - ChIP, CUT&RUN and CUT&Tag Grade

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