Anti-GATA4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-GATA4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT6676

Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity).

Overview

Reactivity: Human Mouse Rat

Tested applications: WB 1:500~1:2000
ICC/IF 1:50~1:200
FC 1:100
ChIP, CUT&RUN and CUT&Tag

Specificity: This antibody recognizes endogenous levels of GATA4 protein.

Properties

Immunogen: A synthesized peptide derived from human GATA4

Clonality: Rabbit Monoclonal, clone number: 7D2

Isotype: Rabbit IgG

Form: Liquid, 50 μl,1mg/ml, PBS (pH 7.2) and 50% Glycerol,0.02% Sodium Azide

Storage instruction: Store at -20°C, Avoid freeze / thaw cycle.

Database links: UniProt : P43694

Host: Rabbit

Applications

WB Image: Western blot – Anti-GATA4 Rabbit mAb (AT6676) at 1/1000 dilution

Sample: in Hela cell lysate

Observed band size: 54 kDa

Recommended secondary antibody: Goat anti rabbit IgG-HRP (Engibody, Catalog number:AT0097)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Anti-GATA4 Rabbit mAb- Staining GATA4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Anti-GATA4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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