Anti-GATA6 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-GATA6 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT7241

GATA-6 (also named GATA-GT1) is a zinc finger transcription activating protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in mesodermally and endodermally-derived tissue. There are two GATA-6 isoforms that arise from alternate start sites: a short form of 449 amino acids (aa), and a long form of 595 aa. The short form of human GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and mouse, respectively.

Overview

Reactivity: Human, mouse, rat

Tested applications: WB 1:500~1:2000
ICC/IF 1:50~1:200
ChIP, CUT&RUN and CUT&Tag

Specificity: This antibody recognizes endogenous levels of GATA6 protein.

Properties

Immunogen: A synthesized peptide derived from human GATA 6

Clonality: Rabbit Monoclonal, clone number: 6Q2

Isotype: Rabbit IgG

Form: Liquid, 50 μl,1mg/ml, PBS (pH 7.2) and 50% Glycerol,0.02% Sodium Azide

Storage instruction: Store at -20°C, Avoid freeze / thaw cycle.

Database links: UniProt : Q92908

Host: Rabbit

Applications

WB Image: Western Blot: Anti-GATA6 Rabbit mAb(AT7241) at 1/1000 dilution

Western blot analysis using GATA6 Rabbit mAb against HEK293 (1) and GATA6 transfected HEK293 (2) cell lysate.

Observed band size: 60 kDa

Recommended secondary antibody: Goat anti rabbit IgG-HRP (Engibody, Catalog number:AT0097)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

ICC/IF Image: Immunocytochemistry:
Anti-GATA6 Rabbit mAb- Staining GATA6 in human cell line CACO-2 shows localization to nucleoplasm.

The nuclear counter stain is DAPI (blue).

Anti-GATA6 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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