Anti-His tag Alpaca-nano™ rabbit monoclonal antibody

Overview

Description

Anti-His tag Alpaca-nano™ rabbit monoclonal antibody (Fab from Alpaca)

Reactivity

Detect transfected 6X His tag fusion proteins expressed in E. coli, yeast, insect cell, mammalian cell, or in vitro transcription/translation systems.

Tested applications

WB    : 1/2000-1/10000.

ELISA    : 1/5000-1/10000.

ICC/IF    : 1/200-1/1000.

IP/CoIP    : 1/100-1/500.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Properties

Immunogen

Synthetic peptide: HHHHHH conjugated to KLH.

Clonality

Monoclonal, clone number: 4D6

Isotype

Fab(VHH domain) of alpaca IgG2c fused to Rabbit IgG Fc    

Storage instruction

Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Conjugation

Unconjugated

Host

Alpaca pacous

Storage buffer

Liquid, 10mM PBS (pH 7.4), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol

Purity

Recombinant expression and Affinity purified

Applications

Highlights

High lot-to-lot consistency

 

Increased sensitivity and higher affinity

 

Animal-free production