Anti-Histone H3 (acetyl K27) antibody - ChIP, CUT&RUN and CUT&Tag Grade

Overview

Description

Anti-Histone H3 (acetyl K27) Rabbit monoclonal antibody - ChIP, CUT&RUN and CUT&Tag Grade

Reactivity

human,mouse,rat and a wide range of species

Tested applications

ChIP: 10-20ul per reaction

CUT&RUN: 2-10ul per reaction

CUT&Tag: 2-10ul per reaction

Specificity

Recognize H3K27ac specifically.

Properties

Immunogen

Synthetic peptide with Histone H3K27ac. The exact sequence is proprietary.

Clonality

Monoclonal, clone number: 5H8

Isotype

rabbit IgG

Form

Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol,0.02% Sodium Azide

Storage instruction

Store at -20°C, Avoid freeze / thaw cycle.

Host

rabbit

Positive Controls

ChIP: Chromatin prepared from HeLa cells.
ChIP-seq: Chromatin prepared from HeLa cells.
CUT&RUN: HeLa cells.

Applications

Application Figure 1

CUT&RUN sequencing - Anti-Histone H3 (acetyl K27) antibody

 

CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 HeLa (Human epithelial cell line from cervix adenocarcinoma) cells and 4 µL of antibody. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. 

Application Figure 2

ChIP-sequencing - Anti-Histone H3 (acetyl K27) antibody

 

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 10 µL of Anti-Histone H3 (acetyl K27) antibody. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.