Anti-Histone H3 (acetyl K9) antibody - ChIP, CUT&RUN and CUT&Tag Grade

Overview

Description

Anti-Histone H3 (acetyl K9) mouse monoclonal antibody - ChIP, CUT&RUN and CUT&Tag Grade

Reactivity

human,mouse,rat and a wide range of species

Tested applications

ChIP: 10-20ul per reaction
CUT&RUN: 2-10ul per reaction
CUT&Tag: 2-10ul per reaction

Specificity

Recognize H3K9ac specifically.

Properties

Immunogen

Synthetic peptide with Histone H3K9ac. The exact sequence is proprietary.

Clonality

Monoclonal, clone number: 6C2

Isotype

mouse IgG

Form

Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol,0.02% Sodium Azide

Storage instruction

Store at -20°C, Avoid freeze / thaw cycle.

Host

Mouse

Positive Controls

WB: HeLa (Human cervix adenocarcinoma epithelial cell), NIH/3T3 (Mouse embryonic fibroblast) and C6 (Rat glial tumor glial cell) treated with 500ng/ml Trichostatin A for 4 hours whole cell lysate IHC-P: Human cerebrum, colorectal carcinoma, mouse colon and rat colon tissue sections. ChIP: Chromatin prepared from HeLa cells. ChIP-seq: Chromatin prepared from HeLa cells. IP: HeLa cells. ICC/IF: HeLa cells. ChIC/CUT&RUN: HeLa cells.

Applications

Application Figure 1

CUT&RUN sequencing - Anti-Histone H3 (acetyl K9) antibody

 

CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human epithelial cell line from cervix adenocarcinoma) cells and 5 µg of antibody. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control is also shown.

Application Figure 2

CUT&RUN sequencing - Anti-Histone H3 (acetyl K9) antibody

 

CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human epithelial cell line from cervix adenocarcinoma) cells and 5 µg of antibody. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control is also shown.

 

Application Figure 3

ChIP-sequencing - Anti-Histone H3 (acetyl K9) antibody

 

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of Anti-Histone H3 (acetyl K9) antibody. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. 

Application Figure 4

CUT&Tag sequencing - Anti-Histone H3 (acetyl K9) antibody

 

CUT&Tag-seq was performed using 200,000 Oli-neu (Oligodendrocyte progenitor) cells.  Cells were permeabilized with 0.05% Digitonin and 0.01% NP-40 for 3 minutes.  A 1:100 dilution of Recombinant Anti-Histone H3 (acetyl K9) antibody was used, along with a rabbit anti-mouse Secondary.  DNA was seg using Illumina NovaSeq S Prime to a depth of 22 million reads.