Anti-Histone H4 (acetyl K16) antibody - ChIP, CUT&RUN and CUT&Tag Grade

Overview

Description

Anti-Histone H4 (acetyl K16) rabbit monoclonal antibody - ChIP, CUT&RUN and CUT&Tag Grade

Reactivity

human,mouse,rat and a wide range of species

Tested applications

ChIP: 10-20ul per reaction

CUT&RUN: 2-10ul per reaction

CUT&Tag: 2-10ul per reaction

Specificity

Recognize H4K16ac specifically.

Properties

Immunogen

Synthetic peptide with Histone H4K16ac. The exact sequence is proprietary.

Clonality

Monoclonal, clone number: 8A1

Isotype

rabbit IgG

Form

Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol,0.02% Sodium Azide

Storage instruction

Store at -20°C, Avoid freeze / thaw cycle.

Host

rabbit

Positive Controls

WB: HeLa, C6 and mouse spleen cell lysates - treated with TSA. IHC-P: Human testis, transitional cell carcinoma and colon tissues. ICC/IF: HeLa cells treated with TSA. Flow Cyt (intra): HeLa cells. CUT&RUN-Seq: HeLa cells.

Applications

Application Figure 1

CUT&RUN sequencing - Anti-Histone H4 (acetyl K16) antibody

 

CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 3 µg of antibody. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads.