Alpaca-HRP-MOU™ Anti-Mouse IgG(H+L) Alpaca Nanobody (HRP) - Co-Incubation of primary antibody and secondary antibody in WB

Product

Alpaca-HRP-MOU™ Anti-Mouse IgG(H+L) Alpaca Nanobody (HRP) - Co-Incubation of primary antibody and secondary antibody in WB

Catalog number :AT8302

This antibody is UNIQUE,
it can be used for Co-Incubation of primary antibody and secondary antibody in WB. it can save 1 hour to 2 hours in WB.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Alpaca immunoglobulin. The size of VHH is extremely small (<15 kDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus, VHH is considered of great value for research, diagnostics and therapeutics.

Anti-Mouse IgG(H+L) Alpaca Nanobody (HRP) is designed for detecting Mouse IgG(H+L). Anti- Mouse IgG(H+L) Alpaca Nanobody (HRP) is based on monovalent, recombinant single domain antibodies coupled to HRP. Based on immunoblot and/or ELISA, Anti- Mouse IgG(H+L) Alpaca Nanobody (HRP) reacts with Mouse IgG(H+L) selectively, no reactivity with rabbit, human, cynomolgus, rat, goat IgG.

Overview

Description: Alpaca-HRP-MOU™ Anti-Mouse IgG(H+L) Alpaca Nanobody (HRP),
this secondary antibody can be co-incubated with the primary antibody at the same time in WB, so this secondary antibody can save WB experiment time of at least one hour.

Reactivity: Mouse IgG(H+L)

Tested applications: ELISA: 1:2000-1:10000
WB: 1:2000-1:10000

Product Picture

Specificity: No cross-reactivity with rabbit, human, cynomolgus, rat, sheep and guinea pig IgG

Properties

Immunogen: Recombinant Mouse IgG

Isotype: VHH domain of alpaca IgG2b

Form: Liquid

Storage instruction: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light, Stable for 12 months at -20°C

Conjugation: HRP

Host: Alpaca pacous

Storage buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol

Purity: Recombinant Expression and Affinity purified

Applications

Highlights: This secondary antibody can be co-incubated with the primary antibody at the same time in WB, so this secondary antibody can save WB experiment time of at least one hour.

Protocols: Benefits Of Alpaca Nanobody

No Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments

Highly specific binding

Low background

High affinity

High stability

Alpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cells

Animal-free production, recombinant expression in yeast and affinity purified

Alpaca-HRP-MOU™ Anti-Mouse IgG(H+L) Alpaca Nanobody (HRP) - Co-Incubation of primary antibody and secondary antibody in WB

Related Products

Reviews