Anti-N1-methyladenosine (m1A) Antibody
Catalog number :AT15111
1-Methyladenosine, also known as m1A, belongs to the class of organic compounds known as purine nucleosides. m1A is important for the tRNA 3-dimensional structure, and it is also important for structure stabilization of tRNA due to its positive electrostatic charge and assists in correct folding of tRNA. Recently evidence suggests that m1A in tRNA is under reversible regulation that is installed by a tRNA m1A methyltransferase (MTase) and is erased by AlkB Homolog 1 (ALKBH1) and AlkB Homolog 3 (ALKBH3).
- Overview
- Description
- Anti-N1-methyladenosine (m1A) Mouse Monoclonal Antibody
- Reactivity
- Species independent
- Tested applications
- MeRIP: 5ug-10ug for 1 ReactionDot blot: 1/1000.ICC: 1/200 - 1/500.IHC: 1/100 - 1/500.Optimal dilutions should be determined by the end user.
- Properties
- Immunogen
- Recombinant protein fused with N1-methyladenosine(m1A).
- Applications
- Application Figure 1
Dot Blot - Anti-1-methyladenosine (m1A) antibody ( antibody dilution: 1/500)
HeLa total RNA 0.5 µg per Dot
- Application Figure 2
meRIP-qPCR
m1A was immunoprecipitated from 20 μg of HeLa polyA+ RNA with 10 μg of AT15111 and 40 μL of Protein A/G Magnetic beads per sample
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