Alpaca-HRP-RAB™ Anti-Rabbit IgG(H+L) Alpaca Nanobody (HRP) - Co-Incubation of primary antibody and secondary antibody in WB

Product

Alpaca-HRP-RAB™ Anti-Rabbit IgG(H+L) Alpaca Nanobody (HRP) - Co-Incubation of primary antibody and secondary antibody in WB

Catalog number :AT8301

This antibody is very UNIQUE,
it can be used for Co-Incubation of primary antibody and secondary antibody in WB. it can save 1 hour to 2 hours in WB.

Rabbit has only one IgG subclass. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 kD and the light chain is about 25 kD. The common IgG is monomeric with a molecular weight of approximately 150 kD.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Alpaca immunoglobulin. The size of VHH is extremely small (<15 kDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus, VHH is considered of great value for research, diagnostics and therapeutics.

Anti-Rabbit IgG(H+L) Alpaca Nanobody (HRP) is designed for detecting rabbit IgG(H+L) . Anti-Rabbit IgG(H+L) Alpaca Nanobody (HRP) is based on monovalent, recombinant single domain antibodies coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-Rabbit IgG(H+L) Alpaca Nanobody (HRP) reacts with rabbit IgG(H+L) selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG.

Overview

Description: Alpaca-HRP-RAB™ Anti-Rabbit IgG(H+L) Alpaca Nanobody (HRP),
this secondary antibody can be co-incubated with the primary antibody at the same time in WB, so this secondary antibody can save WB experiment time of at least one hour.

Reactivity: Rabbit IgG(H+L)

Tested applications: ELISA: 1:2000-1:10000

WB: 1:2000-1:10000

Product Picture

Specificity: No cross-reactivity with mouse, human, cynomolgus, rat, sheep and guinea pig IgG

Properties

Immunogen: Recombinant Rabbit IgG

Isotype: VHH domain of alpaca IgG2b

Form: Liquid

Storage instruction: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light, Stable for 12 months at -20°C

Conjugation: HRP

Host: Alpaca pacous

Storage buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol

Purity: Recombinant Expression and Affinity purified

Applications

Highlights: This secondary antibody can be co-incubated with the primary antibody at the same time in WB, so this secondary antibody can save WB experiment time of at least one hour.

Protocols: Benefits Of Alpaca Nanobody

No Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments

Highly specific binding

Low background

High affinity

High stability

Alpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cells

Animal-free production, recombinant expression in yeast and affinity purified

Alpaca-HRP-RAB™ Anti-Rabbit IgG(H+L) Alpaca Nanobody (HRP) - Co-Incubation of primary antibody and secondary antibody in WB

Related Products

Reviews