Anti-S tag Rabbit mAb

Catalog number :AT1961

S-tag is a 15 amino acid truncated form of the S-peptide, KETAAAKFERQHMDS, used in a variety of protein expression

applications in mammalian cells. Its small size and excessive solubility have made the S-tag an excellent fusion partner in the

production of recombinant proteins. S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). The

N-terminus of the original RNase A, also known as S-peptide, consists of 20 amino acid residues, of which only the first 15 are

required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag. On DNA-level the S-tag can be

attached to the N- or C-terminus of any protein. After gene expression, such a tagged protein can be detected by commercially

available antibodies.

Overview

Reactivity: Anti-S tag Rabbit mAb detects transfected S tag fusion proteins expressed in E.

coli, yeast, insect, mammalian, or in vitro transcription/translation systems

Tested applications: WB : 1/1000-1/2000.

IHC : 1/100-1/500.

ICC/IF : 1/100-1/500.

IP : 1/50-1/100.

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects recombinant proteins containing the S epitope tag. The

antibody recognizes the S-tag fused to either the amino or carboxy terminus of

targeted proteins in transfected cells.

Sequence: KETAAAKFERQHMDS

Properties

Storage instruction: Store at -20°C, Avoid freeze / thaw cycle. Do not aliquot the antibody.

Storage buffer: In 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 1 mg/mL

(0.1% w/v) BSA as a stabilizer and 0.02% (w/v) sodium azide as preservative

Applications

WB Image: All lanes: Anti-S tag antibody (AT1961) at 1/1000 dilution

Lane 1: Multi-tag fusion protein at 0.01 µg

Lane 2: Multi-tag fusion protein at 0.005 µg

Recommended secondary antibody: Goat anti Rabbit IgG-HRP

(Engibody, Catalog number:AT0097) at 1/5000 dilution

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent

HRP Substrate (Engibody, Catalog number: IF6747)