Anti-SOX1 Rabbit mAb

Product

Anti-SOX1 Rabbit mAb

Catalog number :AT5602

Sox (Sry-related box) genes encode a family of transcription factors that play a myriad of roles in developmental processes. Sox1 and Sox2 are the earliest markers that identify neuroectodermal tissue, and these markers, together with Sox9 collectively mark neuronal stem cells (NSC's) that are present in neurogenically active areas of the mature rodent brain. Sox1 is thought to maintain the cell cycle and promote self renewal in NSCs.

Overview

Reactivity: Human Mouse

Tested applications: WB 1:1000~1:2000
IHC 1:50~1:200
ICC/IF 1:50~1:200

Specificity: This antibody recognizes endogenous levels of SOX1 protein.

Properties

Immunogen: A synthesized peptide derived from human SOX1

Clonality: Rabbit Monoclonal, clone number: 4G2

Isotype: Rabbit IgG

Form: Liquid, 50 μl,1mg/ml, PBS (pH 7.2) and 50% Glycerol,0.02% Sodium Azide

Storage instruction: Store at -20°C, Avoid freeze / thaw cycle.

Database links: UniProt : O00570

Host: Rabbit

Applications

WB Image: Western blot – Anti-SOX1 Rabbit mAb (AT5602) at 1/1000 dilution

Sample: in Hela cell lysate

Observed band size: 39 kDa

Recommended secondary antibody: Goat anti rabbit IgG-HRP (Engibody, Catalog number:AT0097)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Anti-SOX1 Rabbit mAb- Staining SOX1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Anti-SOX1 Rabbit mAb

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