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    Anti-Aromatase Rabbit mAb

    Catalog number :AT0461
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    Aromatase is a member of the cytochrome P450 superfamily of enzymes, which are monooxygenases that catalyze reactions involved in drug metabolism and cholesterol and steroid synthesis. Aromatase is responsible for the conversion of testosterone into 17-β estradiol. Aromatase is mainly expressed in the brain, ovaries, and placenta. Aromatase plays an important role in development of the central nervous system during ontogenesis, gonadal development, and sex differentiation. Research studies have suggested that inhibition of aromatase may be an effective therapeutic strategy for postmenopausal breast cancers that are estrogen receptor positive. Mutations in the corresponding aromatase gene are associated with cases of aromatase excess syndrome (AEXS) and aromatase deficiency (AROD) disorders.
    Overview
    Reactivity
    Mouse, Rat, Human
    Tested applications
    Western Blot: 1:1000
    Immunohistochemistry: 1:500
    Immunoprecipitation: 1:100
    Specificity
    Recognizes endogenous levels of total aromatase protein.
    Properties
    Immunogen
    A synthetic peptide made to a C-terminal portion of the human Aromatase protein (between residues 400-502). [UniProt# P11511]
    Clonality
    Monoclonal, clone number: 42A9
    Isotype
    Rabbit IgG
    Storage instruction
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C 
    Applications
    WB Image

    Western Blot: Aromatase Antibody - Detection of aromatase in human fetal temporal lobe lysate using NB 100-1596. ECL exposure
    ICC/IF Image

    Immunocytochemistry/Immunofluorescence: Aromatase Antibody - Aromatase antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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