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    Anti-Aurora A Rabbit mAb - Centrosome Marker

    Catalog number :AT0798
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    Background: 
    Aurora kinases belong to a highly conserved family of mitotic serine/threonine kinases with three members identified among mammals: Aurora A, B, and C. Studies on the temporal expression pattern and subcellular localization of Aurora kinases in mitotic cells suggest an association with mitotic structure. Aurora kinase functional influences span from G2 phase to cytokinesis and may be involved in key cell cycle events such as centrosome duplication, chromosome bi-orientation and segregation, cleavage furrow positioning, and ingression. Aurora A is detected at the centrosomes, along mitotic spindle microtubules, and in the cytoplasm of mitotically proliferating cells. Aurora A protein levels are low during G1 and S phases and peak during the G2/M phase of the cell cycle. Phosphorylation of Aurora A at Thr288 in its catalytic domain increases kinase activity. Aurora A is involved in centrosome separation, maturation, and spindle assembly and stability. Expression of Aurora B protein also peaks during the G2/M phase of the cell cycle; Aurora B kinase activity peaks at the transition from metaphase to the end of mitosis. Aurora B associates with chromosomes during prophase prior to relocalizing to the spindle at anaphase. Aurora B regulates chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. Expression of both Aurora A and Aurora B during the G2/M phase transition is tightly coordinated with histone H3 phosphorylation; research investigators have observed overexpression of these kinases in a variety of human cancers. Aurora C localizes to the centrosome from anaphase to cytokinesis and both mRNA and protein levels peak during G2/M phase. Although typical Aurora C expression is limited to the testis, research studies report overexpression of Aurora C is detected in various cancer cell lines.
     
    UniProt ID: O14965
    Overview
    Reactivity
    Human, Mouse, Rat, Bovine, Pig, Monkey
    Tested applications
    Western Blotting: 1/1000-1/2000
    Immunofluorescence: 1/500
    Immunohistochemistry (Paraffin): 1/500
    Immunoprecipitation(IP) : 1/100 
    Optimal dilutions/concentrations should be determined by the end user. 
    Specificity
    This antibody detects endogenous levels of total Aurora A protein. 
    Properties
    Immunogen
    a synthetic peptide corresponding to residues surrounding Pro381 of human Aurora A protein
    Clonality
    Monoclonal, clone number: 3E7
    Isotype
    Rabbit IgG
    Form
    Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol,0.02% Sodium Azide
    Storage instruction
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.
    Host
    Rabbit
    Applications
    WB Image

    Western Blot: Aurora A Antibody - WB detection of Aurora A in HeLa whole cell lysate.
    ICC/IF Image

    Immunocytochemistry/Immunofluorescence: Aurora A Antibody - IF Confocal analysis of HeLa cells using Aurora A antibody (1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

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