Anti-Aurora B Rabbit mAb - Centrosome Marker

Overview

Reactivity

Human, Mouse, Rat, Bovine, Pig, Monkey

Tested applications

Western Blotting: 1/1000-1/2000

Immunofluorescence: 1/500

Immunohistochemistry (Paraffin): 1/500

Immunoprecipitation(IP) : 1/100 

Optimal dilutions/concentrations should be determined by the end user. 

Specificity

This antibody detects endogenous levels of total Aurora B protein. 

Properties

Immunogen

a synthetic peptide corresponding to the amino terminus of human Aurora B/AIM1. Antibodies are purified by protein A and peptide affinity chromatography.

Clonality

Monoclonal, clone number: 2G14

Isotype

Rabbit IgG

Form

Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host

Rabbit

Applications

WB Image

Western Blot: Aurora B Antibody - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with this antibody. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. this antibody binds strongly to Aurora B at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with this antibody.

ICC/IF Image

Immunocytochemistry/Immunofluorescence: Aurora B Antibody - HeLa cell cultures were stained with this antibody(green). Localization of Aurora B is cell cycle phase dependent. First, Aurora B staining was seen in chromosome arm in cells in prophase (green small dots). During anaphase, Aurora B localized in midzone and then concentrated in midbodies between the two daughter cells during telophase. It is therefore a useful marker of midbodies and dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (red). Blue is a DNA stain.

Immunocytochemistry/Immunofluorescence: Aurora B Antibody - Analysis of HeLa cells stained with antibody to aurora B kinase, dilution 1:1000 in red, and costained with chicken pAb to vimentin, dilution 1:1000 in green. Blue is DAPI staining of nuclear DNA. this antibody produces strong staining associated with chromosomes in prophase (A), the centromere in prometaphase and metaphase (B), the central mitotic spindle in anaphase (C), and midbodies between the two daughter cells during telophase and beyond (D). The vimentin antibody stains the intermediate filament network in these cells.