Anti-BACE1 Rabbit mAb

Product

Anti-BACE1 Rabbit mAb

Catalog number :AT0443

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.

Overview

Description: Rabbit monoclonal antibody to BACE1

Reactivity: Mouse, Rat, Human

Tested applications: WB: Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa. We recommend overnight incubation at 4C and using 5% skim milk to block.

ICC: Use a concentration of 10 µg/ml.

ICC/IF: Use at an assay dependent concentration.

Properties

Immunogen: Synthetic peptide, corresponding to amino acids 485-501 of Human BACE. The peptide sequence differs fromthose of mouse and rat BACE by one amino acid (1).

Clonality: Monoclonal, clone number: 4F3

Isotype: Rabbit IgG

Form: Liquid

Storage instruction: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Database links

Applications

WB Image: All lanes : Anti-BACE1 antibody (AT0443) at 1 µg/ml

Lane 1 : Human brain tissue lysate with absence of blocking peptide
Lane 2 : Human brain tissue lysate with BACE1 peptide
Lane 3 : Mouse 3T3 cell lysate

Observed band size : 70 kDa

ICC/IF Image: AT0443 staining BACE1 in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with AT0443 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.

Anti-BACE1 Rabbit mAb

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