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    Anti-Bacteria GAPDH Mouse mAb - Loading Control

    Catalog number :AT1749
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    GAPDH is a critical enzyme in the glocolytic pathway, catalyzing the 6th step of glycolysis which is the beginning of the pay-off phase of glycolysis where a gain of ATP can be seen. GAPDH is primarily associated with metabolic functions but has also been shown to have a role in transcription activation, apoptosis and intracellular vesicle shuttling. Due to the importance of GAPDH in metabolic processes it is expressed in nearly all cells and therefore serves as a good loading control target. 
    Overview
    Reactivity
    Bacterial Strain BL21
    Tested applications
    WB (1:2000)
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user. 
    Specificity
    GAPDH Antibody detects endogenous levels of GAPDH in Bacteria lysates.
    Properties
    Immunogen
    This mouse monoclonal antibody is produced by immunizing mouse with Full length native protein from Bacteria.
    Clonality
    8B7
    Isotype
    Mouse IgG1
    Form
    Liquid, 100 μg at 1mg/ml
    Storage instruction
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C 
    Applications
    WB Image

    All lanes: Anti-GAPDH antibody at 1:1000 
     
    Lane 1: Bacterial Strain BL21 lysate
    Predicted band size: 37 kDa

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