Anti-Bcl-xL Rabbit mAb

Overview

Reactivity

Human, Mouse, Rat, Bovine, Pig, Monkey

Tested applications

Western Blotting: 1/1000-1/2000

Immunofluorescence: 1/500

Immunohistochemistry (Paraffin): 1/500

Immunoprecipitation(IP) : 1/100 

Optimal dilutions/concentrations should be determined by the end user. 

Specificity

This antibody detects endogenous levels of total Bcl-xL protein. The antibody does not cross-react with other Bcl-2 family members.

Properties

Immunogen

a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL.

Clonality

Monoclonal, clone number: 4D8

Isotype

Rabbit IgG

Form

Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host

Rabbit

Applications

WB Image

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, C6 rat glioma cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Bcl xL Antibody followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for Bcl xL at approximately 25 kDa (as indicated).

IHC Image

 Immunohistochemistry-Paraffin: Bcl-xL Antibody - Analysis of paraffin-embedded human colon cancer tissue using anti-Bcl-XL antibody. Counter stained with hematoxylin.

ICC/IF Image

Immunocytochemistry/Immunofluorescence: Bcl-xL Antibody - Staining Bcl-XL in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.