Anti-CD133 Rabbit mAb

Overview

Reactivity

Human, mouse, rat, bovine, chicken, Drosophilia melanogaster

Tested applications

Western Blotting 1:1000

Immunofluorescence  1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP)  1;100 

Optimal dilutions/concentrations should be determined by the end user. 

 

Specificity

This antibody detects endogenous levels of total CD133 protein. This antibody is not sensitive to the glycosylation status of CD133.

Properties

Immunogen

This antibody is produced by immunizing animals with recombinant protein specific to the 1st extracellular loop of human CD133 protein. The epitope has been mapped to a region spanning amino acid residues 303-312.

Clonality

Monoclonal, clone number: 5BC2

Isotype

Rabbit IgG

Form

Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host

Rabbit

Applications

WB Image

Western Blot: CD133 Antibody - Western Blot image of anti-human CD133. Total protein from HeLa cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1：1000 anti-hCD133 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

ICC/IF Image

Immunocytochemistry/Immunofluorescence: CD133 Antibody - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-hCD133 at a 1:200 dilution overnight at 4C and detected with and anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.