Anti-CD34 Rabbit mAb

Product

Anti-CD34 Rabbit mAb

Catalog number :AT0704

CD34 is a type I transmembrane glycophosphoprotein expressed by hematopoietic stem/progenitor cells, vascular endothelium and some fibroblasts. CD34 expression has been the hallmark used to identify hematopoietic stem cells for many years. CD34+ hematopoietic stem cells expand and differentiate into all the lymphohematopoietic lineages upon cytokine or growth factor stimulation and lose CD34 expression upon differentiation. However, recent studies performed in various laboratories conflict with that convention. The extracellular domain of CD34 is homologous to CD43, a protein involved in cell-cell adhesion, and CD34 has been shown to function as a negative regulator of cell adhesion. CD34 associates with CrkL but not CrkII, is a substrate for PKC, and activation of PKC is coupled with surface expression of CD34.

UniProt ID:

P28906

Overview

Reactivity: Human, mouse, rat, Xenopus laevis, bovine, chicken, Drosophilia melanogaster

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1;100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects endogenous levels of total CD34 protein.

Properties

Immunogen: This antibody was developed against a recombinant protein

Clonality: Monoclonal, clone number: 6B7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western blot shows lysates of mouse brain (cortex) tissue and rat testis tissue. PVDF Membrane was probed with 1:1000 of CD34 Antigen Affinity-purified Rabbit Polyclonal Antibody (Catalog: AT0704) followed by HRP-conjugated Anti-rabbit IgG Secondary Antibody (Catalog: AT0097). A specific band was detected for CD34 at approximately 85 90 kDa (as indicated). This experiment was conducted under reducing conditions.

IHC Image: Immunohistochemistry-Paraffin: CD34 Antibody - Staining of human kidney shows moderate to strong membranous positivity in endothelial cells.

Immunohistochemistry-Paraffin: CD34 Antibody - Staining of human testis shows moderate to strong membranous positivity in endothelial cells

Anti-CD34 Rabbit mAb

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