Anti-CD63 Rabbit mAb - Exosome Marker

Product

Anti-CD63 Rabbit mAb - Exosome Marker

Catalog number :AT1609

Background:

CD63 belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin enriched microdomains (TEMs) where they mediate a range of processes including adhesion, motility, membrane organization, and signal transduction. CD63, like other tetraspanins, is enriched in exosomes. It is also a component of Weibel-Palade bodies found in endothelial cells. Research studies demonstrate several functions of CD63 in different cell types including roles in mast cell degranulation, VEGF signaling in endothelial cells, recruitment of leukocytes to endothelial cells, and endosomal sorting during melanogenesis.

UniProt ID: P08962

Overview

Reactivity: Human, Mouse, Rat, Bovine, Pig, Monkey

Tested applications: Western Blotting: 1/1000-1/2000

Immunofluorescence: 1/500

Immunohistochemistry (Paraffin): 1/500

Immunoprecipitation(IP) : 1/100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects endogenous levels of total CD63 protein.

Properties

Immunogen: Human CD63 aa 100-200

Clonality: Monoclonal, clone number: 4B32

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: CD63 Antibody - Analysis of CD63 on different lysates using anti-CD63 antibody at 1/1,000 dilution. Positive control: Lane 1: HL60 Lane 2: THP-1 Lane 3: NIH/3T3

IHC Image: Immunohistochemistry-Paraffin: CD63 Antibody - Analysis of paraffin-embedded human lung cancer tissue using anti-CD63 antibody. Counter stained with hematoxylin.

Anti-CD63 Rabbit mAb - Exosome Marker

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