Anti-CDK4 Rabbit mAb

Product

Anti-CDK4 Rabbit mAb

Catalog number :AT0386

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Overview

Description: Rabbit monoclonal antibody to CDK4

Reactivity: Reacts with: Mouse, Human, Rat, Pig

Tested applications

WB	: 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
ICC/IF	: 1/100 - 1/1000.
IHC-P	: 1/100 - 1/1000.

Properties

Immunogen: Recombinant fragment, corresponding to a region within amino acids 148-303 of Human Cdk4 (Uniprot ID: P11802).

Clonality: Monoclonal, clone number: D1A7

Isotype: IgG

Form: Liquid
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol

Storage instruction: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Database links

Applications

WB Image: Anti-Cdk4 antibody (AT0386) at 1/1000 dilution + 293T whole cell lysate at 30 µg

Predicted band size : 34 kDa

IHC Image: Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue, labelling Cdk4 using AT0386 at 1/500 dilution.

ICC/IF Image: Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling Cdk4 with AT0386 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

Anti-CDK4 Rabbit mAb

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