Anti-CXCR4 Rabbit mAb

Product

Anti-CXCR4 Rabbit mAb

Catalog number :AT0452

CXCR4 is a chemokine receptor that belongs to the G protein-coupled receptor family. It is activated by a small cytokine, CXCL12, also known as stromal cell derived factor 1 (SDF-1). The main function of CXCR4 is the mediation of the homing of progenitor cells in the bone marrow and their recruitment to sites of injury. More recently, CXCR4 has been studied, as a potential therapeutic target, in the context of autoimmune diseases as well as cancer, as the receptor is involved in the regulation of migration, proliferation, and survival of cancer cells.

Overview

Reactivity: Mouse, Rat, Human

Tested applications: Western Blot: 1:1000

Immunohistochemistry: 1:500

Immunoprecipitation: 1:100

Specificity: Recognizes endogenous levels of total CXCR4 protein.

Properties

Immunogen: Rabbit anti-CXCR4 polyclonal antibody was raised against a peptide corresponding to amino acids 328-338 of human CXCR4.

Clonality: Monoclonal, clone number: 6D7

Isotype: Rabbit IgG

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C

Applications

WB Image: Western Blot: CXCR4 Antibody - Detection of 15 ug in HeLa cells with antibody concentration at 1 ug/ml.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: CXCR4 Antibody - Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-CXCR4 (AT0452) at a 1:50 dilution for 1 hour at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Anti-CXCR4 Rabbit mAb

Related Products

Reviews