Anti-CXCR4 Rabbit mAb

Product

Anti-CXCR4 Rabbit mAb

Catalog number :AT0760

Background:

CXCR4 is a chemokine receptor that belongs to the G protein-coupled receptor family. It is activated by a small cytokine, CXCL12, also known as stromal cell derived factor 1 (SDF-1). The main function of CXCR4 is the mediation of the homing of progenitor cells in the bone marrow and their recruitment to sites of injury. More recently, CXCR4 has been studied, as a potential therapeutic target, in the context of autoimmune diseases as well as cancer, as the receptor is involved in the regulation of migration, proliferation, and survival of cancer cells.

UniProt ID: P61073

Overview

Reactivity: Human, mouse, rat, bovine, pig

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1:100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total CXCR4 protein.

Properties

Immunogen: recombinant protein specific to the extracellular domains of human CXCR4 protein.

Clonality: Monoclonal, clone number: 5C7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: CXCR4 Antibody - WB analysis of CXCR4 in A. HeLa and B. A431 cell lysate.

IHC Image: CXCR4 was detected in perfusion fixed frozen sections of mouse spleen using Anti-CXCR4 Antibody at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes.

ICC/IF Image: CXCR4 was detected in immersion fixed mouse splenocytes using Anti-CXCR4 Antibody at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.

Anti-CXCR4 Rabbit mAb

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