Anti-Calbindin Rabbit mAb

Product

Anti-Calbindin Rabbit mAb

Catalog number :AT0694

Calcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, parvalbumin and troponin C are members of the low molecular weight calcium-binding protein family. Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity.

Overview

Reactivity: Human, mouse, rat, Xenopus laevis, bovine, chicken, Drosophilia melanogaster

Tested applications: Western Blotting 1:1000

Immunoprecipitation 1:200

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total Calbindin.

Properties

Immunogen: Synthetic peptide within Human Calbindin aa 150-250. The exact sequence is proprietary.

Database link: P05937

Clonality: Monoclonal, clone number: 4E1

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Calbindin Antibody - Analysis of Calbindin on different lysates using anti-Calbindin antibody at 1/1,000 dilution.
Lane 1: Rat brain

Lane 2: Mouse kidney

IHC Image: Immunohistochemistry-Paraffin: Calbindin Antibody - Analysis of paraffin-embedded human kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Calbindin Antibody - Staining Calbindin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Anti-Calbindin Rabbit mAb

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