Anti-Calreticulin Rabbit mAb

Product

Anti-Calreticulin Rabbit mAb - ER Marker

Catalog number :AT0794

Background:

Calcium is a universal signaling molecule involved in many cellular functions such as cell motility, metabolism, protein modification, protein folding, and apoptosis. Calcium is stored in the endoplasmic reticulum (ER), where it is buffered by calcium binding chaperones such as calnexin and calreticulin, and is released via the IP3 Receptor channel. Calreticulin also functions as an ER chaperone that ensures proper folding and quality control of newly synthesized glycoproteins. As such, calreticulin presumably does not alter protein folding but regulates proper timing for efficient folding and subunit assembly. Furthermore, calreticulin retains proteins in non-native conformation within the ER and targets them for degradation.

UniProt ID: P27797

Overview

Reactivity: Human, Mouse, Rat, Bovine, Pig, Monkey

Tested applications: Western Blotting: 1/1000-1/2000

Immunofluorescence: 1/500

Immunohistochemistry (Paraffin): 1/500

Immunoprecipitation(IP) : 1/100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects endogenous levels of total Calreticulin protein.

Properties

Immunogen: a synthetic peptide corresponding to residues near the amino terminus of human calreticulin protein.

Clonality: Monoclonal, clone number: 4GV2

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Calreticulin Antibody - Human kidney lysate.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Calreticulin Antibody - The Calreticulin antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

Anti-Calreticulin Rabbit mAb - ER Marker

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