Anti-Caspase 12 Rabbit mAb

Product

Anti-Caspase 12 Rabbit mAb

Catalog number :AT0774

Background:

Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. One of the mechanisms for caspase-12 activation is related to calpain mediated cleavage at T132 and K158, both of which are located at the amino-terminal region of the caspase-12. Caspase-12 also has a putative caspase cleavage site located at the carboxy-terminal region of the protein. In cortical neurons, caspase-12 is involved at least partially in the amyloid-beta neurotoxicity process.

UniProt ID: O08736

Overview

Reactivity: Human, Mouse, Rat, Bovine, Pig, Monkey

Tested applications: Western Blotting: 1/1000

Immunofluorescence: 1/500

Immunohistochemistry (Paraffin): 1/500

Immunoprecipitation(IP) : 1/100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of full-length caspase-12 protein (55 kDa) and its cleaved product (42 kDa). The antibody does not cross-react with other caspases.

Properties

Immunogen: a synthetic peptide corresponding to residues surrounding amino acid 158 of mouse caspase-12. Antibodies are purified by protein A and peptide affinity chromatography.

Clonality: Monoclonal, clone number: 4E9

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Caspase-12 Antibody - Analysis of Caspase-12 in (A) human spleen and (B) mouse spleen tissue lysate with Caspase-12 antibody at 1 ug/mL.

ICC/IF Image: Immunofluorescence: Caspase-12 Antibody - Mouse Liver cells with Caspase-12 antibody at 10 ug/mL.

Anti-Caspase 12 Rabbit mAb

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