Anti-Caspase 3 (active/cleaved) Rabbit mAb

Product

Anti-Caspase 3 (active/cleaved) Rabbit mAb

Catalog number :AT0770

Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.

S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

Overview

Description: anti Caspase 3 Rabbit monoclonal antibody

Reactivity: Mouse, Rat, Rabbit, Human, Monkey, Pig

Properties

Immunogen: Recombinant full length protein corresponding to Human Caspase-3.

Database link: P42574

Clonality: Polyclonal

Isotype: Monoclonal, clone number: 2B7

Form: Liquid

Storage instruction: Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Applications

WB Image: Western blot analysis of caspase-3 precursor expression in HuT 78 (A) and CCRF-HSB-2 (B) whole cell lysates.

ICC/IF Image: Caspase 3 Antibody in IF

Immunofluorescence analysis of Caspase 3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Caspase-3 Antibody at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti Rabbit IgG Secondary Antibody at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin Panels d is a merged image showing cytoplasmic localization and panel e a is no primary antibody control. The images were captured using a Nikon microscope at 20X magnification.

Anti-Caspase 3 (active/cleaved) Rabbit mAb

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