Anti-Caspase 9 Rabbit mAb

Product

Anti-Caspase 9 Rabbit mAb

Catalog number :AT0378

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Overview

Description: Rabbit monoclonal antibody to Caspase 9, No cross-reactivity to other members of the caspase family.

Reactivity: Mouse, Human, Rat

Tested applications

ICC/IF	: Use a concentration of 20 µg/ml.
ICC	: Use a concentration of 2 µg/ml.
WB	: 1/1000 - 1/2000.
IHC-P	: Use at an assay dependent dilution.

Properties

Immunogen: Peptide sequence corresponding to amino acids 299 to 318 of human Caspase 9.

Clonality: Monoclonal, clone number: 3G8

Isotype: IgG

Form: Liquid
Preservative: 0.02% Sodium Azide
Constituents: PBS

Storage instruction: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Database links

Applications

WB Image

Anti-Caspase 9 antibody (AT0378) at 1/1000 dilution + HeLa whole cell lysate.

Observed band size : 46 kDa

Why is the actual band size different from the predicted?

Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include...

post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein
post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases
splice variants - alternative splicing may create different sized proteins from the same gene
relative charge - the composition of amino acids (charged vs non-charged)
multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands

IHC Image: Immunocytochemistry - Caspase 9 antibody

AT0378 at 2µg/ml staining Caspase 9 in K562 cells by Immunocytochemistry.

ICC/IF Image: Immunocytochemistry/ Immunofluorescence-Anti-Caspase 9 antibody

Immunofluorescence of Caspase-9 in K562 cells using AT0378 at 20 ug/ml.

Anti-Caspase 9 Rabbit mAb

Related Products

Reviews