Anti-Cleaved PARP1 Rabbit mAb

Overview

Reactivity

Human, Mouse, Rat

Tested applications

Western Blotting: 1/500-1/2000

Immunofluorescence: 1/50-1/200

Immunohistochemistry (Paraffin): 1/50-1/200

Optimal dilutions/concentrations should be determined by the end user. 

Specificity

This antibody detects endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. The antibody does not recognize  the small fragment (24 kDa) of human PARP1 protein .

Properties

Immunogen

A synthetic peptide corresponding to residues containing in the large fragment (89 kDa) of human Cleaved PARP1 protein produced by caspase cleavage

Clonality

Monoclonal, clone number: 4C1

Isotype

Rabbit IgG

Form

Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction

Store at -20°C

Host

Rabbit

Applications

WB Figure 1

All lanes use the Antibody at 1:1000 dilution for 1 hour at room temperature.

WB Figure 2

All lanes use the Antibody at 1:3000 dilution for 1 hour at room temperature.

WB Figure 3

All lanes use the Antibody at 1:3000 dilution for 1 hour at room temperature.

ICC/IF Image

Immunofluorescent analysis of HeLa cells, using Cleaved PARP Antibody.

Application Figure 1

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle - gastrocnemius , using the Antibody at 1:50 dilution.

Application Figure 2

Immunohistochemical analysis of paraffin-embedded Human colon, using the Antibody at 1:50 dilution.

Application Figure 3

Immunohistochemical analysis of paraffin-embedded Rat skin, using the Antibody at 1:50 dilution.