Anti-Cofilin Rabbit mAb

Product

Anti-Cofilin Rabbit mAb

Catalog number :AT0695

Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration. In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments. The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin. Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm.

Overview

Reactivity: Human, mouse, rat, Xenopus laevis, bovine, chicken, Drosophilia melanogaster

Tested applications: Western Blotting 1:1000

Immunoprecipitation 1:200

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total Cofilin protein.

Properties

Immunogen: Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Cofilin.

Clonality: Monoclonal, clone number: 4T21

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Cofilin Antibody - Analysis of extracts from COLO 205 cells using cofilin antibody (Lane 1 and 2) and cofilin (phospho-Ser3) antibody (Lane 3 and 4).

IHC Image: Immunohistochemistry: Cofilin Antibody - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin antibody.

Anti-Cofilin Rabbit mAb

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