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    Anti-Collagen I Rabbit mAb

    Catalog number :AT0709
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    Type 1 collagen is the most abundant collagen in many human tissues, including bone, skin, and tendons. It is a trimeric complex comprised of two molecules of COL1A1 (alpha-1 type 1 collagen) and one molecule of COL1A2 (alpha-2 type 1 collagen). The expression levels of COL1A1 are regulated by multiple mechanisms, including mRNA stability, translation, and posttranslational modification. Overexpression of COL1A1 has been positively associated with tissue fibrosis disorders, including systemic sclerosis, while loss-of-function mutations in the COL1A1 gene are a major causative factor for osteogenesis imperfecta (brittle bone disease). Notably, COL1A1 expression levels have also been associated with tumor development in gastric, lung, thyroid, and breast cancers. Research studies suggest that upregulation of COL1A1 can generate a modified extracellular matrix environment that promotes cancer cell survival, proliferation, metastasis, and invasion.
    Collagen-1 (also known as collagen alpha, COL1A1, and alpha-1 type I collagen) is the largest component of fibrillar collagen found in cartilage and connective tissues, and synthesizes by fibroblasts, osteoblasts, and odontoblasts. Trimers are formed by combing two alpha one chains and one alpha 2 chain. Mutations of the gene are found in Ehlers-Danlos syndrome, as well as osteogenesis imperfecta. Over 50 mutations have been found in these diseases, including deletions, insertions, splice variants, and substitutions.
     
    UniProt ID: 
    P02452
     
    Overview
    Reactivity
    Human, mouse, rat, bovine, chicken, Drosophilia melanogaster
    Tested applications
    Western Blotting 1:1000
    Immunofluorescence  1:500
    Immunohistochemistry (Paraffin) 1:500
    Immunoprecipitation(IP)  1;100 
    Optimal dilutions/concentrations should be determined by the end user. 
     
    Specificity
    This antibody detects endogenous levels of total Collagen I protein.
    Properties
    Immunogen
    A synthetic peptide made to an internal portion of the human Collagen I protein (between residues 150-200)
    Clonality
    Monoclonal, clone number: 3G21
    Isotype
    Rabbit IgG
    Form
    Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol,0.02% Sodium Azide
    Storage instruction
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.
    Host
    Rabbit
    Applications
    WB Image

    Western Blot: Collagen I alpha 1 Antibody - Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1,000 overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20,00 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.
    IHC Image

    Immunohistochemistry: Collagen I Antibody - Analysis of Collagen 1 in mouse epidermis using DAB with hematoxylin counterstain.
    ICC/IF Image

    Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody - Collagen 1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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