Anti-Collagen IV Rabbit mAb

Overview

Reactivity

Human, mouse, rat, bovine, chicken, Drosophilia melanogaster

Tested applications

Western Blotting 1:1000

Immunofluorescence  1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP)  1;100 

Optimal dilutions/concentrations should be determined by the end user. 

 

Specificity

This antibody detects endogenous levels of total Collagen IV protein.

Properties

Immunogen

Full length native protein (purified) corresponding to Collagen IV. Collagen Type IV from human and bovine placenta. The immunogen maintains the native conformation of the protein.

Clonality

Monoclonal, clone number: 6H7

Isotype

Rabbit IgG

Form

Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host

Rabbit

Applications

WB Image

Western blot shows lysates of human placenta tissue. PVDF Membrane was probed with 1:1000 of Collagen IV alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog: AT0710) followed by HRP-conjugated Anti-rabbit IgG Secondary Antibody (Catalog: AT0097). A specific band was detected for Collagen IV a1 at approximately 95 kDa (as indicated) ref 1. This experiment was conducted under reducing conditions.

ICC/IF Image

Collagen IV alpha 1 was detected in immersion fixed THP 1 human acute monocytic leukemia cell line using Collagen IV alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog: AT0710) at 1:500 for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.