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    Anti-Cyclin B1 Rabbit mAb

    Catalog number :AT0815
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    Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C. Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis. While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147. At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression. Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers.
    Overview
    Reactivity
    Mouse, Rat, Human
    Tested applications
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunohistochemistry (Paraffin) 1:500
    Optimal dilutions/concentrations should be determined by the end user. 
    Specificity
    This antibody recognizes endogenous levels of total cyclin B1 protein. This antibody also detects a 100 kDa protein of unknown origin in some cell lines.
    Properties
    Immunogen
    Synthetic peptide within Human Cyclin B1 aa 400-500 (C terminal). The exact sequence is proprietary.
    Clonality
    Monoclonal, clone number: 5H7
    Isotype
    Rabbit IgG
    Form
    Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol,0.02% Sodium Azide
    Storage instruction
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.
    Host
    Rabbit
    Applications
    WB Image

    Western Blot: Cyclin B1 Antibody - Analysis of Cyclin B1 on different lysates using anti-Cyclin B1 antibody at 1/1,000 dilution. 
    Lane 1: Hela Lane 2: Daudi Lane 3: K562
     
    IHC Image

    Immunohistochemistry-Paraffin: Cyclin B1 Antibody - Analysis of paraffin-embedded human tonsil tissue using anti-Cyclin B1 antibody. Counter stained with hematoxylin.

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