Anti-Cyclin B1 Rabbit mAb
Catalog number :AT0815
Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C. Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis. While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147. At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression. Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers.
- Overview
- Reactivity
- Mouse, Rat, Human
- Tested applications
- Western Blotting 1:1000Immunoprecipitation 1:100Immunohistochemistry (Paraffin) 1:500Optimal dilutions/concentrations should be determined by the end user.
- Specificity
- This antibody recognizes endogenous levels of total cyclin B1 protein. This antibody also detects a 100 kDa protein of unknown origin in some cell lines.
- Properties
- Immunogen
- Synthetic peptide within Human Cyclin B1 aa 400-500 (C terminal). The exact sequence is proprietary.
- Clonality
- Monoclonal, clone number: 5H7
- Isotype
- Rabbit IgG
- Form
- Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol,0.02% Sodium Azide
- Storage instruction
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.
- Host
- Rabbit
- Applications
- WB Image
Western Blot: Cyclin B1 Antibody - Analysis of Cyclin B1 on different lysates using anti-Cyclin B1 antibody at 1/1,000 dilution.Lane 1: Hela Lane 2: Daudi Lane 3: K562
- IHC Image
Immunohistochemistry-Paraffin: Cyclin B1 Antibody - Analysis of paraffin-embedded human tonsil tissue using anti-Cyclin B1 antibody. Counter stained with hematoxylin.
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