Anti-Cyclin D1 Rabbit mAb

Product

Anti-Cyclin D1 Rabbit mAb

Catalog number :AT0767

Background:

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase. The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression. Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation.

UniProt ID: P24385

Overview

Reactivity: Human, Mouse, Rat, Bovine, Pig, Monkey

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1:100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total Cyclin D1 protein.

Properties

Immunogen: a synthetic peptide corresponding to the carboxy-terminus of human cyclin D1.

Clonality: Monoclonal, clone number: 3B4

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Cyclin D1 Antibody - Analysis of Cyclin D1 on different lysates using anti-Cyclin D1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12 Lane 3: SH-SY-5Y

IHC Image: Immunohistochemistry-Paraffin: Cyclin D1 Antibody - Analysis showing staining in the nucleus of human tonsil tissue (right) compared with a negative control in the absence of primary antibody (left).

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody - Analysis of Cyclin D1 (green) showing staining in the nucleus of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cyclin D1 antibody in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.

Anti-Cyclin D1 Rabbit mAb

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