Anti-Cytochrome C Rabbit mAb

Product

Anti-Cytochrome C Rabbit mAb

Catalog number :AT0609

Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form. This event further triggers caspase-3 activation and eventually leads to apoptosis.

Overview

Reactivity: Mouse, Rat, Human

Tested applications: Western Blot 1:1000-1:5000

Immunocytochemistry/Immunofluorescence 1:50-1:200

Immunohistochemistry 1:50-1:200

Immunoprecipitation 1:10 - 1:500

Specificity: This antibody detects endogenous levels of total cytochrome c protein.

Properties

Immunogen: Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Cytochrome C.

Clonality: Monoclonal, clone number: 6K7C

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C

Applications

WB Image: Western Blot: Cytochrome c Antibody (AT0609) - Analysis of Cytochrome C on different lysates using anti-Cytochrome C antibody at 1/1,000 dilution.

Lane 1: Mouse kidney Lane 2: Rat kidney

IHC Image: Immunohistochemistry-Paraffin: Cytochrome c Antibody (AT0609) - Analysis of paraffin-embedded human liver tissue using anti-Cytochrome C antibody. Counter stained with hematoxylin.

ICC/IF Image: Cytochrome c Antibody (AT0609) - Staining Cytochrome C in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Anti-Cytochrome C Rabbit mAb

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