Anti-DYKDDDDK (FLAG®) tag Mouse mAb (HRP)

Overview

Description

anti-Flag-Tag mouse mAb Conjugated HRP - (Binds to same epitope as Sigma's Anti-FLAG M2 Antibody)

Reactivity

All species

Tested applications

WB (1:5000-1:10000)
ELISA (1:5000-1:10000)

Optimal dilutions/concentrations should be determined by the end user.

Product Picture

Properties

Immunogen

Synthetic peptide: DYKDDDDK conjugated to KLH

Clonality

3A6

Isotype

Mouse IgG1

Form

Liquid, 100 μg at 1mg/ml

Storage instruction

Aliquot and store at -20°C

Conjugation

Horseradish peroxidase (HRP)

Applications

WB Image

Western blot 

Lane 1 : Negative control 

Lane 2 : HEK293T cell lysate transfected with Flag-Tag fusion protein

 

Predicted band size : 32 kDa

HEK293T cell lysates were generated from transient transfection of the vector containing Flag-Tag