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    Dextrin Agarose Resin 6FF for MBP-Tagged Protein Purification

    Catalog number :P4160
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    Dextrin Beads Resin 6FF is an affinity media for purification of proteins tagged with Maltose Binding protein (MBP).
    Dextrin Agarose Resin is a chromatography medium for purifying proteins fused to maltose binding protein (MBP-tagged protein). Recombinant proteins with MBP-tags often gives increased expression levels and higher solubility of the target protein. Proper folding of the attached protein has also been shown to be promoted by the MBP tag. Since MBP increase the solubility, the tag is extremely useful for recombinant proteins accumulated in an insoluble form (inclusion bodies).
    Dextrin is the polymeric product resulted by enzymatic hydrolysis of amylose with beta amylase. Compared to Amylose that is susceptible to amylase degradation, Dextrin is very stable. When used as the ligand substrate immobilized on the resin for purification of the MBP tag, Dextrin offers consistently strong affinity absorption even after repeated uses.
    The base matrix of Dextrin Agarose Resin is a robust, highly cross- linked 6% agarose. The crosslinking of the base matrix has been optimized to give high physical and chemical stability, high specificity of the binding.
    Affinity purification using Dextrin Agarose Resin take place under physiological conditions and mild elution is performed using maltose which preserve target protein activity. These mild conditions may even allow purification of intact protein complexes.
    Our proprietary coupling technology ensures stable immobilization of active ligand and minimal ligand leakage in a wide range of purification buffer conditions. The resins can be reused multiple times to purify the same target protein.
    Overview
    Tested applications
    Dextrin Beads 6FF provides convenient affinity purification of recombinant Proteins tagged with maltose binding Protein (MBP). This robust and stable Dextrin Beads 6FF based medium enables elution of target Protein in narrow peaks, which minimizes the need for further concentration steps.
    Product Picture
    Dextrin Agarose Resin 6FF for MBP
    Properties
    Storage instruction
    2°C to 8°C in 20% ethanol
    Beads Diameter
    90 µm (45 μm - 165 μm)
    Binding Capacity
    Approx. 7 mg MBP2*-paramyosin-δ-Sal/ml medium (Mr ~70 000,  multimer in solution)
    Approx. 16 mg MBP2*-β-galactosidase/ml medium (Mr ~158 000,  multimer in solution)
    Ligand
    Dextrin
    Matrix
    6% highly cross-linked agarose supplied as 50% slurry
    Maximum pressure
    0.3 MPa, 3 bar
    Chemical stability
    Stable in all commonly used aqueous buffers, 0.5 M NaOH  (regeneration and cleaning)
    Flow rate
    Recommended flow velocity ≤ 150 cm/h, Maximum flow velocity < 300 cm/h
    pH stability
    working range > 7
    short-term:2 to 13
    Applications
    Highlights
    • Dextrin Beads 6FF is a robust, high resolution medium based on the 90 μm agarose matrix
     
    • The small, evenly sized beads ensure that MBP-tagged Proteins elute in narrow peaks, thus minimizing the need for further concentration steps
     
    • Physiological conditions and mild elution preserve target Protein activity
     
    • Dextrin Beads 6FF tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same column to be used for repeated purifications

    • Stable coupling of ligand, low leakage; can be re-used to purify the same protein

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