Dextrin Agarose Resin is a chromatography medium for purifying proteins fused to maltose binding protein (MBP-tagged protein). Recombinant proteins with MBP-tags often gives increased expression levels and higher solubility of the target protein. Proper folding of the attached protein has also been shown to be promoted by the MBP tag. Since MBP increase the solubility, the tag is extremely useful for recombinant proteins accumulated in an insoluble form (inclusion bodies).
Dextrin is the polymeric product resulted by enzymatic hydrolysis of amylose with beta amylase. Compared to Amylose that is susceptible to amylase degradation, Dextrin is very stable. When used as the ligand substrate immobilized on the resin for purification of the MBP tag, Dextrin offers consistently strong affinity absorption even after repeated uses.
The base matrix of Dextrin Agarose Resin is a robust, highly cross- linked 6% agarose. The crosslinking of the base matrix has been optimized to give high physical and chemical stability, high specificity of the binding.
Affinity purification using Dextrin Agarose Resin take place under physiological conditions and mild elution is performed using maltose which preserve target protein activity. These mild conditions may even allow purification of intact protein complexes.
Our proprietary coupling technology ensures stable immobilization of active ligand and minimal ligand leakage in a wide range of purification buffer conditions. The resins can be reused multiple times to purify the same target protein.